Journal: BioMed Research International

Article Title: Elastase and Cathepsin G from Primed Leukocytes Cleave Vascular Endothelial Cadherin in Hemodialysis Patients

doi: 10.1155/2014/459640

Figure Lengend Snippet: Soluble VE-cadherin fragments (140 and 100 kDa forms) in HD plasma versus NC plasma.Proteins of plasma samples depleted from albumin and immunoglobulins of NC subjects and HD patient were separated on SDS-PAGE followed by western blot analysis (a) Representative gels showing three sizes of VE-cadherin: the whole molecule: 140 kDa and the cleaved forms: 100 and 40 kDa in NC (1,3) and HD plasma (2,4). (b) Relative densities of VE-cadherin from NC subjects and HD patient plasma (* P > 0.05 HD versus NC; n = 10).

Article Snippet: The whole 140 kDa molecule and the 100 kDa fragment were detected using mouse anti-human VE-cadherin (Cell Signaling Technology, MA, USA), while the 40 kDa fragment was detected using mouse anti-human VE-cadherin (United States Biological, Massachusetts, USA), which is not able to recognize the 140 kDa and the 100 kDa molecules.

Techniques: SDS Page, Western Blot

Journal: BioMed Research International

Article Title: Elastase and Cathepsin G from Primed Leukocytes Cleave Vascular Endothelial Cadherin in Hemodialysis Patients

doi: 10.1155/2014/459640

Figure Lengend Snippet: (a) Positive correlations between plasma active cathepsin G in plasma of NC (●) and HD patients (▲) and soluble VE-cadherin 100 kDa fragment ( r = 0.51; P < 0.05; continuous line). (b) Positive correlations between plasma active elastase in plasma of NC and HD patients and VE-cadherin 100 kDa fragment ( r = 0.53; P < 0.05; continuous line).

Article Snippet: The whole 140 kDa molecule and the 100 kDa fragment were detected using mouse anti-human VE-cadherin (Cell Signaling Technology, MA, USA), while the 40 kDa fragment was detected using mouse anti-human VE-cadherin (United States Biological, Massachusetts, USA), which is not able to recognize the 140 kDa and the 100 kDa molecules.

Techniques:

Journal: BioMed Research International

Article Title: Elastase and Cathepsin G from Primed Leukocytes Cleave Vascular Endothelial Cadherin in Hemodialysis Patients

doi: 10.1155/2014/459640

Figure Lengend Snippet: Indirect immunohistochemical staining of VE-cadherin (40 kDa form) in HUVEC after cocultivation with PMNLs and after exposure to elastase and cathepsin G (light microscopy, magnification ×20).

Article Snippet: The whole 140 kDa molecule and the 100 kDa fragment were detected using mouse anti-human VE-cadherin (Cell Signaling Technology, MA, USA), while the 40 kDa fragment was detected using mouse anti-human VE-cadherin (United States Biological, Massachusetts, USA), which is not able to recognize the 140 kDa and the 100 kDa molecules.

Techniques: Immunohistochemical staining, Staining, Light Microscopy

Journal: BioMed Research International

Article Title: Elastase and Cathepsin G from Primed Leukocytes Cleave Vascular Endothelial Cadherin in Hemodialysis Patients

doi: 10.1155/2014/459640

Figure Lengend Snippet: A representative gel showing soluble VE-cadherin (100 kDa form) in PMNLs and HUVEC cocultivation media separated by SDS-PAGE followed by western blot analysis.

Article Snippet: The whole 140 kDa molecule and the 100 kDa fragment were detected using mouse anti-human VE-cadherin (Cell Signaling Technology, MA, USA), while the 40 kDa fragment was detected using mouse anti-human VE-cadherin (United States Biological, Massachusetts, USA), which is not able to recognize the 140 kDa and the 100 kDa molecules.

Techniques: SDS Page, Western Blot